1. Cultivation materials: The cultivation materials are mainly cotton husk, bran, corn cob and rice bran, which account for about 90% of the total. Other auxiliary materials such as corn flour, superphosphate and calcium carbonate account for 10%, and the moisture content of the cultivation material. Adjust 60%~65%.
2, bottling, sterilization and cooling: using mechanical mixing and bottling, mixing requirements are uniform, each bottle is filled with 850 grams of raw materials, the surface is compacted, a hole in the middle of the bottle, and covered with a filter cover to ensure the bacteria The nutrient source and air for the normal growth of silk.
3. Inoculation: The cultivated strains will be sterilized by mechanical automatic inoculation under the aseptic condition, the inoculum volume is 10 g/bottle, and the 850 ml strain bottle can be connected to 80-90 bottles.
4. Mycelial culture: The inoculated flask is cultured in a dark room under a certain temperature, humidity and culture time. Flammulina velutipes requires high light and is sensitive and must be cultured in a dark room.
5, sputum bacteria: After the hyphae are full, carry out sputum operation. That is, the cap is removed, and the old bacteria in the mouth of the bottle is removed by a sterilizing machine.
6, bud: after the sputum directly into the bud room for bud, technical parameters: daytime temperature 13 ° C ~ 14 ° C, night temperature 10 ° C ~ 12 ° C, relative humidity 90%, time is about 8 days.
7. Inhibition: After completion of the bud, it is sent to the antibacterial chamber for inhibition culture. Technical parameters: temperature 3 °C ~ 5 °C, relative humidity 80% ~ 85%, time 5 ~ 7 days, while appropriate ventilation, every 4 Ventilation for 15 minutes. After 5~7 hours, the obvious cap and stipe can grow. And pay attention to the light can not be strong, in order to improve the quality of the fruiting body.
8. Growth: When the cup grows out of the bottle by 1 cm, adjust the room temperature to 6 ° C ~ 8 ° C, and carry out light treatment to improve the quality of fruiting bodies. When the fruit body grows 3~4 cm from the mouth of the bottle, it is necessary to cover the paper (plastic) tube in time.
9. Harvesting: When the stipe is 13~15 cm and the cap diameter is about 1 cm, it can be harvested. Two to three days before harvesting, the fan is turned on to allow the water to evaporate, so that the relative humidity of the cultivation room is maintained at 70% to 80%, which can improve product quality and extend shelf life.
10. Packing and fresh-keeping: The harvested mushroom body should be placed separately according to different grade requirements, and the connecting part of the base of the stipe and the medium should be removed, and the packaging of different sizes should be carried out according to market requirements, and the bag should be pumped with polyethylene film. Airtight, refrigerated at low temperature after packaging.
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